Generation of High-Affinity Recombinant Antibody Fragments for Peptide Enrichment Immuno-MRM Assays
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چکیده
protein quantitation that obtains precise and sensitive measurements of specific target analytes in complex biological samples. Prior to performing MRM, many peptide analytes require an enrichment step to achieve sufficient sensitivity in the assay. Coupling a peptide immunoaffinity enrichment step to MRM (i.e. an immuno-MRM assay) is effective for most analytes and provides high sensitivity and specificity, a wide dynamic range, and convenience of sample handling when measuring endogenous proteins. Furthermore, immuno-MRM is reproducible across laboratories and capable of multiplexing several analytes simultaneously.
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